Optimization of phycocyanin extraction from Spirulina platensis using factorial design

被引：188
|
作者：
Silveira, S. T. [1 ]
Burkert, J. F. M. [1 ]
Costa, J. A. V. [1 ]
Burkert, C. A. V. [1 ]
Kalil, S. J. [1 ]
机构：
[1] Fundacao Univ Fed Rio Grande, Dept Chem, BR-96201900 Rio Grande, RS, Brazil
来源：
BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 08期
关键词：
extraction; optimization; phycocyanin; Spirulina platensis;
D O I：
10.1016/j.biortech.2006.05.050
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Phycocyanin extraction from cyanobacteria Spirulina platensis was optimized using factorial design and response surface techniques. The effects of temperature and biomass-solvent ratio on phycocyanin concentration and extract purity were evaluated to determine the optimum conditions for phycocyanin extraction. The optimum conditions for the extraction of phycocyanin from S. platensis were the highest biomass-solvent ratio, 0.08 g mL(-1), and 25 degrees C. Under these conditions it's possible to obtain an extract of phycocyanin with a concentration of 3.68 mg mL(-1) and purity ratio (A(615)/A(280)) of 0.46. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1629 / 1634
页数：6
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