Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit

被引：0
|
作者：
Lin, Meiai [1 ]
Zhang, Shuyu [1 ]
Liu, Qiao [2 ]
Shao, Changshun [2 ]
Su, Xuantao [1 ]
机构：
[1] Shandong Univ, Sch Control Sci & Engn, Inst Biomed Engn, Jinan 250061, Shandong, Peoples R China
[2] Shandong Univ, Sch Basic Med Sci, Dept Mol Med & Genet, Jinan 250012, Shandong, Peoples R China
来源：
14TH NATIONAL CONFERENCE ON LASER TECHNOLOGY AND OPTOELECTRONICS (LTO 2019) | 2019年 / 11170卷
基金：
中国国家自然科学基金;
关键词：
Microfluidic cytometry; light sheet; label free; light scattering; tumor cell senescence; single cell analysis; LEUKEMIC-CELLS; CLASSIFICATION;
D O I：
10.1117/12.2534145
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
The detection of senescent cells becomes increasing important for tumor therapy and drug screening. Here a light sheet microfluidic cytometer with a disposable hydrodynamic focusing unit is developed for two dimensional (2D) light scattering measurements of single cells. The mixed polystyrene microspheres of 3.87 and 2.0 mu m in diameter are successfully differentiated by our 2D light scattering microfluidic cytometer. The application of the 2D light scattering microfluidic cytometry for the label-free analysis of senescent cells without any labeling or staining is demonstrated by measurements of H2O2-treated U87 cells. Our light sheet-based 2D light scattering microfluidic cytometer is easy to assemble with a disposable hydrodynamic unit, which may find wild applications in clinics for label-free cell classification.
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页数：6
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