Extractive cultivation of xylanase by Penicillium janthinellum in a poly(ethylene glycol) cashew-nut tree gum aqueous two-phase system

被引：6
|
作者：
Oliveira, LA [1 ]
Neto, BB
Porto, ALF
Tambourgi, EB
机构：
[1] Univ Estadual Campinas, Dept Sistemas Quim & Informat, LIKA, BR-13081970 Campinas, SP, Brazil
[2] Univ Fed Pernambuco, Dept Quim Fundamental, Recife, PE, Brazil
[3] UNIT, ITP, BR-49032490 Aracaju, SE, Brazil
来源：
BIOTECHNOLOGY PROGRESS | 2004年 / 20卷 / 06期
关键词：
D O I：
10.1021/bp049746h
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cultivation of the fungus Penicilliumjanthinellum for xylanase production was studied in a poly(ethylene glycol)/cashew-nut tree gum aqueous two-phase system, using a two-level fractional factorial design. The parameters studied were initial pH, cultivation time, type of agro-industrial residue (oat husk or corn cob), agitation, temperature, and phase-forming polymers. The xylanase produced during fermentation partitioned into the top phase. The agitation and temperature (negative), cultivation time and initial pH (positive) effects proved statistically significant for xylanase production. The highest percentage yield of the xylanase in the top and its production in the top phase, about 97% and 160.7 U/mL, were obtained in cultures of 120 h, 40 rpm, 25 degreesC, and pH 5.0.
引用
收藏
页码：1880 / 1884
页数：5
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