Molecular characterisation and diagnostics of some gall-forming nematodes of the family Anguinidae Nicoll, 1935 (Nematoda: Tylenchida) using COI mtDNA

被引：0

作者：

Roubtsova, Tatiana, V ^{[1
]}

Burbridge, Julie ^{[2
]}

Subbotin, Sergei A. ^{[2
,3
]}

机构：

[1] Univ Calif Davis, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA

[2] Calif Dept Food & Agr, Plant Pest Diagnost Ctr, 3294 Meadowview Rd, Sacramento, CA 95832 USA

[3] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Leninskii Prospect 33, Moscow 119071, Russia

来源：

RUSSIAN JOURNAL OF NEMATOLOGY | 2020年 / 28卷 / 02期

关键词：

Afrina sporoboliae; Anguina agrostis; Anguina danthoniae; Anguina graminis; Anguina tritici; Anguina sp; PCR; Real-time PCR; PLANT-PARASITIC NEMATODES; PCR; IDENTIFICATION; PHYLOGENY; AGROSTIS;

D O I：

10.24411/0869-6918-2020-10013

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Several nematodes of the family Anguinidae inducing galls on grasses were characterised in this study. Sequences of partial CO/ gene were obtained from Afrina sp. from Astrebra pectinata and Anguina agrostis, A. danthoniae, A. graminis, A. tritici and Anguina sp. from Dactylis glomerata. Conventional PCR with species-specific primers were developed for rapid detection of Afrina sporoboliae, Anguina tritici, A. danthoniae and Anguina sp. from Dactylis glomerata. Real-time PCR with TaqMan probes were also designed for detection of specimens of Afrina sporoboliae and A. danthoniae. Real-time PCR assays were able reliably to detect DNA extracted from less than half of nematode specimens per a single tube.

引用

页码：123 / 130

页数：8

共 8 条

[1] Molecular characterisation of gall-forming nematodes, Mesoanguina amsinckiae and Anguina danthoniae (Anguinidae: Tylenchida) from California, USA
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[7] Molecular characterisation and diagnostics of some Longidorus species (Nematoda: Longidoridae) from Russia and other countries using rRNA genes
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[8] Molecular characterisation and diagnostics of some Longidorus species (Nematoda: Longidoridae) from Russia and other countries using rRNA genes
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European Journal of Plant Pathology, 2014, 138 : 377 - 390

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