PHOSPHOLIPASE, PROTEINASE AND HEMOLYTIC ACTIVITIES OF CANDIDA ALBICANS ISOLATES OBTAINED FROM CLINICAL SPECIMENS

This study was aimed to determine the phospholipase, proteinase and hemolytic activities of Candida albicans strains isolated from clinical specimens. A total of 147 C. albicans strains isolated from blood (n= 29), respiratory specimens (n= 44), urine (n= 52), pus (n= 17) and stool (n= 5) were included in the study. Proteinase and phospholipase activities were determined in 81 % and 76% of C.albicans isolates, respectively. All C.albicans isolates revealed beta-hemolytic activity on Sabouraud dextrose agar supplemented with 7% fresh sheep blood and 3% glucose. Phospholipase and proteinase positivity were highest among the respiratory isolates. Proteinase activity of respiratory (93%) and blood (83%) isolates were statistically significantly higher than that of urine (77%; p= 0.032), pus (65%; p= 0.007) and stool isolates (60%; p= 0.026). While phospholipase activity showed statistically significant difference between respiratory (84%) and pus (53%) isolates (p= 0.014), no statistically significant difference was determined for blood (79%), urine (75%) and stool (80%) isolates (p> 0.05). Two blood isolates with 4+ proteinase activity and 3 urine isolates with 3+ proteinase activity were phospholipase negative. One urine isolate with 4+ phospholipase activity and 4 with 3+ phospholipase activity were proteinase negative. Phospholipase and proteinase negative 1 isolate from stool and 1 isolate from pus were found to have 4+ hemolytic activity. In conclusion, besides proteinase and phospholipase enzyme activities, hemolytic activity may play an important role for the Calbicons infections. The pathogenetic role of these virulence factors should be evaluated by further clinical studies.