Identification and discrimination of Burkholderia pseudomallei, B-mallei, and B-thailandensis by real-time PCR targeting type III secretion system genes

被引：81

作者：

Thibault, FM ^{[1
]}

Valade, E ^{[1
]}

Vidal, DR ^{[1
]}

机构：

[1] Ctr Rech, Serv Sante Armees, F-38702 La Tronche, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 12期

关键词：

D O I：

10.1128/JCM.42.12.5871-5874.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

引用

页码：5871 / 5874

页数：4

共 15 条

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Rainbow, L
Hart, CA
Winstanley, C
JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 51 (05) : 374 - 384
[2] BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
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PLOS ONE, 2010, 5 (11):
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Lowe, Chinn-Woan
Satterfield, Benjamin A.
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PLOS ONE, 2016, 11 (10):
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Novak, RT
Glass, MB
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Currie, BJ
Wilkins, PP
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (01) : 85 - 90
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U'Ren, JM
Van Ert, MN
Schupp, JM
Easterday, WR
Simonson, TS
Okinaka, RT
Pearson, T
Keim, P
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (11) : 5771 - 5774
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Scholz, HC
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Treu, TM
Neubauer, H
DIAGNOSTIC MOLECULAR PATHOLOGY, 2004, 13 (04) : 247 - 253
[7] Development of a 5′-nuclease real-time PCR assay targeting fliP for the rapid identification of Burkholderia mallei in clinical samples
Tomaso, H
Scholz, HC
Al Dahouk, S
Eickhoff, M
Treu, TM
Wernery, R
Wernery, U
Neubauer, H
CLINICAL CHEMISTRY, 2006, 52 (02) : 307 - 310
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不详
INFECTION, 2005, 33 (03) : 207 - 208
[9] Development of Hydrolysis Probe-Based Real-Time PCR for Identification of Virulent Gene Targets of Burkholderia pseudomallei and B. mallei-A Retrospective Study on Archival Cases of Service Members with Melioidosis and Glanders
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MILITARY MEDICINE, 2012, 177 (02) : 216 - 221
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Meumann, Ella M.
Novak, Ryan T.
Gal, Daniel
Kaestli, Mirjam E.
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Spencer, Emma
Glass, Mindy B.
Gee, Jay E.
Wilkins, Patricia P.
Currie, Bart J.
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) : 3028 - 3030

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