Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine

被引：88

作者：

Watanabe, Yasunori ^{[1
,2
]}

Mendonca, Luiza ^{[3
]}

Allen, Elizabeth R. ^{[4
]}

Howe, Andrew ^{[5
]}

Lee, Mercede ^{[6
]}

Allen, Joel D. ^{[1
]}

Chawla, Himanshi ^{[1
]}

Pulido, David ^{[4
]}

Donnellan, Francesca ^{[4
]}

Davies, Hannah ^{[4
]}

Ulaszewska, Marta ^{[4
]}

Belij-Rammerstorfer, Sandra ^{[4
,7
]}

Morris, Susan ^{[4
]}

Krebs, Anna-Sophia ^{[3
]}

Dejnirattisai, Wanwisa ^{[6
]}

Mongkolsapaya, Juthathip ^{[8
,9
]}

Supasa, Piyada ^{[6
]}

Screaton, Gavin R. ^{[6
,10
]}

Green, Catherine M. ^{[6
]}

Lambe, Teresa ^{[4
,7
]}

Zhang, Peijun ^{[3
,5
]}

Gilbert, Sarah C. ^{[4
,7
]}

Crispin, Max ^{[1
]}

机构：

[1] Univ Southampton, Sch Biol Sci, Southampton SO17 1BJ, Hants, England

[2] Univ Oxford, Oxford Glycobiol Inst, Dept Biochem, Oxford OX1 3QU, England

[3] Univ Oxford, Nuffield Dept Med, Div Struct Biol, Oxford OX3 7BN, England

[4] Univ Oxford, Jenner Inst, Nuffield Dept Med, Oxford, England

[5] Diamond Light Source, Electron Bioimaging Ctr, Didcot OX11 0DE, Oxon, England

[6] Univ Oxford, Wellcome Ctr Human Genet, Oxford OX3 7BN, England

[7] NIHR Oxford Biomed Res Ctr, Oxford, England

[8] Univ Oxford, Chinese Acad Med Sci CAMS Oxford Inst COI, Oxford OX3 7BN, England

[9] Mahidol Univ, Siriraj Hosp, Fac Med, Dengue Hemorrhag Fever Res Unit,Off Res & Dev, Bangkok, Thailand

[10] Univ Oxford, John Radcliffe Hosp, Div Med Sci, Oxford OX3 7BN, England

来源：

ACS CENTRAL SCIENCE | 2021年 / 7卷 / 04期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会; 英国惠康基金;

关键词：

CRYO-EM; GLYCOSYLATION; VISUALIZATION; CORONAVIRUS;

D O I：

10.1021/acscentsci.1c00080

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation, and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirm the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.

引用

页码：594 / 602

页数：9

共 50 条

[1] ChAdOx1 nCoV-19 (AZD1222) vaccine candidate significantly reduces SARS-CoV-2 shedding in ferrets
Marsh, Glenn A.
McAuley, Alexander J.
Au, Gough G.
Riddell, Sarah
Layton, Daniel
Singanallur, Nagendrakumar B.
Layton, Rachel
Payne, Jean
Durr, Peter A.
Bender, Hannah
Barr, Jennifer A.
Bingham, John
Boyd, Victoria
Brown, Sheree
Bruce, Matthew P.
Burkett, Kathie
Eastwood, Teresa
Edwards, Sarah
Gough, Tamara
Halpin, Kim
Harper, Jenni
Holmes, Clare
Horman, William S. J.
van Vuren, Petrus Jansen
Lowther, Suzanne
Maynard, Kate
McAuley, Kristen D.
Neave, Matthew J.
Poole, Timothy
Rootes, Christina
Rowe, Brenton
Soldani, Elisha
Stevens, Vittoria
Stewart, Cameron R.
Suen, Willy W.
Tachedjian, Mary
Todd, Shawn
Trinidad, Lee
Walter, Duane
Watson, Naomi
Drew, Trevor W.
Gilbert, Sarah C.
Lambe, Teresa
Vasan, S. S.
[J]. NPJ VACCINES, 2021, 6 (01)
[2] ChAdOx1 nCoV-19 (AZD1222) vaccine candidate significantly reduces SARS-CoV-2 shedding in ferrets
Glenn A. Marsh
Alexander J. McAuley
Gough G. Au
Sarah Riddell
Daniel Layton
Nagendrakumar B. Singanallur
Rachel Layton
Jean Payne
Peter A. Durr
Hannah Bender
Jennifer A. Barr
John Bingham
Victoria Boyd
Sheree Brown
Matthew P. Bruce
Kathie Burkett
Teresa Eastwood
Sarah Edwards
Tamara Gough
Kim Halpin
Jenni Harper
Clare Holmes
William S. J. Horman
Petrus Jansen van Vuren
Suzanne Lowther
Kate Maynard
Kristen D. McAuley
Matthew J. Neave
Timothy Poole
Christina Rootes
Brenton Rowe
Elisha Soldani
Vittoria Stevens
Cameron R. Stewart
Willy W. Suen
Mary Tachedjian
Shawn Todd
Lee Trinidad
Duane Walter
Naomi Watson
Trevor W. Drew
Sarah C. Gilbert
Teresa Lambe
S. S. Vasan
[J]. npj Vaccines, 6
[3] Efficacy of ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV-2 lineages circulating in Brazil
Sue Ann Costa Clemens
Pedro M. Folegatti
Katherine R. W. Emary
Lily Yin Weckx
Jeremy Ratcliff
Sagida Bibi
Ana Verena De Almeida Mendes
Eveline Pipolo Milan
Ana Pittella
Alexandre V. Schwarzbold
Eduardo Sprinz
Parvinder K. Aley
David Bonsall
Christophe Fraser
Michelle Fuskova
Sarah C. Gilbert
Daniel Jenkin
Sarah Kelly
Simon Kerridge
Teresa Lambe
Natalie G. Marchevsky
Yama F. Mujadidi
Emma Plested
Maheshi N. Ramasamy
Peter Simmonds
Tanya Golubchik
Merryn Voysey
Andrew J. Pollard
[J]. Nature Communications, 12
[4] AZD1222 (ChAdOx1 nCoV-19) Vaccine: Hurdles and Visions
Ahmad, Md. Altamash
Kaur, Harleen
Kumari, Pratima
Singh, Ravinder
Kaur, Rupinder
Chopra, Hitesh
Sardana, Ojus
Bin Emran, Talha
Dhama, Kuldeep
[J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2023, 17 (01): : 12 - 22
[5] Efficacy of ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV-2 lineages circulating in Brazil
Clemens, Sue Ann Costa
Folegatti, Pedro M.
Emary, Katherine R. W.
Weckx, Lily Yin
Ratcliff, Jeremy
Bibi, Sagida
Mendes, Ana Verena De Almeida
Milan, Eveline Pipolo
Pittella, Ana
Schwarzbold, Alexandre, V
Sprinz, Eduardo
Aley, Parvinder K.
Bonsall, David
Fraser, Christophe
Fuskova, Michelle
Gilbert, Sarah C.
Jenkin, Daniel
Kelly, Sarah
Kerridge, Simon
Lambe, Teresa
Marchevsky, Natalie G.
Mujadidi, Yama F.
Plested, Emma
Ramasamy, Maheshi N.
Simmonds, Peter
Golubchik, Tanya
Voysey, Merryn
Pollard, Andrew J.
[J]. NATURE COMMUNICATIONS, 2021, 12 (01)
[6] SARS-CoV-2 Spike Protein Expression In Vitro and Hematologic Effects in Mice Vaccinated With AZD1222 (ChAdOx1 nCoV-19)
Stebbings, Richard
Jones, Christopher
Cotton, Peter
Armour, Gillian
Maguire, Shaun
Skellett, Vicky
Tang, Chi-Man
Goodman, Joanne
Brady, Tyler
Takahashi, Virginia
Daunt, Andrew
Lapointe, Jean-Martin
Cohen, Taylor S.
[J]. FRONTIERS IN IMMUNOLOGY, 2022, 13
[7] ChAdOx1 nCoV-19 vaccine for SARS-CoV-2
Lodge, Archie
[J]. LANCET, 2020, 396 (10261): : 1486 - 1486
[8] Risk Factors for Grade 3 to Grade 4 Adverse Reactions to the ChAdOx1 nCoV-19 Vaccine (AZD1222) Against SARS-CoV-2
Lee, Sang Won
Lee, Hyun
Lee, Sun-Kyung
Moon, Ji-Yong
Moon, SeolHwa
Chung, Sung Jun
Yeo, Yoomi
Park, Tai Sun
Park, Dong Won
Kim, Tae-Hyung
Sohn, Jang Won
Yoon, Ho Joo
Kim, Sang-Heon
[J]. FRONTIERS IN MEDICINE, 2021, 8
[9] AZ arm: Delayed cutaneous reaction to ChAdOx1 nCoV-19 (AZD1222) vaccine
Wei-Kai, Hung
Chi, Ching-Chi
Wang, Shu-Hui
[J]. DERMATOLOGICA SINICA, 2022, 40 (01) : 52 - 53
[10] Antibody Response to ChAdOx1 nCoV-19 (AZD1222) Vaccine in Kidney Transplant Recipients
Bhadauria, Dharmendra S.
Katiyar, Harshita
Goel, Amit
Tiwari, Prachi
Kishore, Ravi V. Krishna
Aggarwal, Amita
Verma, Alka
Khetan, Dheeraj
Kaul, Anupma
Yachha, Monika
Behera, Manas Ranjan
Yadav, Brijesh
Prasad, Narayan
[J]. VACCINES, 2022, 10 (10)

← 1 2 3 4 5 →