In Vitro Root and Shoot Formation of Rosa centifolia using Plant Growth Regulators

Rosa centifolia is one of the most economically important floriculture crops that is grown and used for oil extract which has various applications in pharmaceutical industries. It is commercially propagated through cuttings, but low rooting percentage has always been a problem since rose species are difficult to root. To address this problem, a protocol was developed to meet the growing demands for R. centifolia through in vitro propagation technique. Nodal segments were used as an explant and were cultured in a MS medium supplemented with different concentrations (0.2, 0.4, 0.6 and 0.8 mu M) of Benzylaminopurine (BAP) and Kinetin (KIN), alone and in combination of both, for induction of shoots. For root induction, different concentrations (0.2, 0.4, and 0.6 mu M) of Indole Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) were used in a 1/2 strength MS medium. BAP at 0.2 mu M concentration produced the highest number of shoots (1.93) and longest shoot elongation (5.55cm) vis-a-vis other concentrations. Moreover, minimum number of days (10.2) to shoot induction was observed at 0.4 mu M of BAP in combination with 0.2 mu M of KIN. On the other hand, IBA at 0.4 mu M had the minimum number of days (10.07) to produce roots, highest number of roots (2.47), and longest root elongation (4.21cm) among other concentrations. Thus, it is concluded that BAP at 0.2 mu M and IBA at 0.4 mu M concentration are effective for shoot and root formation of R. centifolia, respectively.