Near-field recording technique for high-resolution fluorescent imaging

被引：2

作者：

Moriguchi, C. ^{[1
]}

Inami, W. ^{[2
,3
]}

Egami, C. ^{[1
,3
]}

Kawata, Y. ^{[1
,3
]}

Terakawa, S. ^{[3
,4
]}

Tsuchimori, M. ^{[5
]}

Watanabe, O. ^{[5
]}

机构：

[1] Shizuoka Univ, Fac Engn, Joho Ku, Hamamatsu, Shizuoka 4328581, Japan

[2] Shizuoka Univ, Div Global Res Leaders, Joho Ku, Hamamatsu, Shizuoka 4328561, Japan

[3] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

[4] Hamamatsu Univ Sch Med, Photon Med Res Ctr, Hamamatsu, Shizuoka 4313192, Japan

[5] Toyota Cent Res & Dev Labs Inc, Aichi 4801192, Japan

来源：

APPLIED PHYSICS LETTERS | 2010年 / 96卷 / 24期

关键词：

biological techniques; cellular biophysics; fluorescence; near-field scanning optical microscopy; POLYMER-FILMS; MICROSCOPY; SPECIMENS; PROBE;

D O I：

10.1063/1.3455095

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We present near-field fluorescent imaging with high resolution using a near-field recording technique. In this technique, optical intensity distribution localized near specimens is recorded as the surface topographic distribution of a photosensitive film. Because the technique does not require the scanning probes for detecting light, it is possible to observe living biological specimens. Fluorescence imaging is one of important tools in investigations of cell structures and functions in biology. We combined near-field recording technique with fluorescence imaging and demonstrated the observations of fluorescent particles beyond the diffraction limit. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3455095]

引用

页数：3

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