Isolation and structure characterization of glucuronoxylans from Dolichos lablab L. hull

被引:10
|
作者
Liu, Dan [1 ]
Tang, Wei [1 ]
Xin, Yue [1 ]
Wang, Ze-Xia [1 ]
Huang, Xiao-Jun [1 ]
Hu, Jie-Lun [1 ]
Yin, Jun-Yi [1 ]
Nie, Shao-Ping [1 ]
Xie, Ming-Yong [1 ,2 ]
机构
[1] Nanchang Univ, State Key Lab Food Sci & Technol, China Canada Joint Lab Food Sci & Technol Nanchan, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China
[2] Jiangxi Normal Univ, Natl R&D Ctr Freshwater Fish Proc, Nanchang 330022, Jiangxi, Peoples R China
关键词
Dolichos lablab L. hull; Glucuronoxylan; Structure; Conformation; Methylation; NMR; PHYSICOCHEMICAL PROPERTIES; EXCHANGE CHROMATOGRAPHY; ACETYLATED HETEROXYLAN; CHAIN CONFORMATION; POLYSACCHARIDE; FEATURES; XYLANS; HYDROLYSIS; SEEDS;
D O I
10.1016/j.ijbiomac.2021.04.033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polysaccharides were extracted by hot water and alkali in sequence from Dolichos lablab L. hull, and further purified by ion-exchange and gel columns. Hot water extracted D. lablab hull polysaccharide (DLHP) was rich in glucuronoxylan and pectin, and alkali extracted polysaccharide (DLHAP) mostly embraced glucuronoxylan. The structures of purified glucuronoxylans from DLHP and DLHAP were mainly analyzed by HPAEC-PAD, methylation combined with GC-MS, NMR and SEC-MALLS. DLHP-1 was identified as acetylated glucuronoxylan containing -> 4)-beta-Xylp-(1 -> backbone with substitution at O-2 site by alpha-GlcpA/4-O-methyl-alpha-GlcpA. The molar ratio of beta-Xylp to alpha-GlcpA was 6.9:1, and acetylation was mainly at O-3 site of beta-Xylp with acetylation degree of 21.5%. DLHP-1 and DLHP-2 had similar physicochemical properties, except for molecular weight (M-w). DLHAP-1 was the non-methylated glucuronoxylan almost without acetylation, and it had the molar ratio of beta-Xylp to alpha-GlcpA of 5.6:1. Besides, DLHP-1 (M-w of 20.0 x 10(3) g mol(-1)) adopted semi-flexible chain, while DLHAP-1 (M-w of 15.4 x 10(3) g mol(-1)) showed flexible chain. These results provided a structural basis for study on polysaccharides from D. lablab hull, which was benefit for understanding biological activities and developing functional food or pharmaceuticals of D. lablab. (C) 2021 Elsevier B.V. All rights reserved.
引用
收藏
页码:1026 / 1036
页数:11
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