High efficiency of genetic transformation and regeneration of Artemisia annua L. via Agrobacterium tumefaciens-mediated procedure

被引：50
|
作者：
Han, JL [1 ]
Wang, H [1 ]
Ye, HC [1 ]
Liu, Y [1 ]
Li, ZQ [1 ]
Zhang, Y [1 ]
Zhang, YS [1 ]
Yan, F [1 ]
Li, GF [1 ]
机构：
[1] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
来源：
PLANT SCIENCE | 2005年 / 168卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Artemisia annua L; Agrobacterium tumefaciens; transformation efficiency; shoot induction frequency; GFP;
D O I：
10.1016/j.plantsci.2004.07.020
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Artemisinin is a new effective antimalarial drug extracted from the medicinal plant Artemisia annua L. There is a great interest in enhancing the production of artemisinin by means of transgenic plants. In this work, experiments with different combinations of hormone concentration were performed to obtain higher frequency of shoot and root induction. In particular, the factors influencing A. tumefaciens-mediated transformation of A. annua were explored to optimize the transformation system, which included A. tumefaciens strain, plant genotype, preculture period, composition of infection bacterium suspension, methods of co-cultivation, and co-cultivation period. Finally, a system of high efficiency of genetic transformation and regeneration of A. annua was established. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：73 / 80
页数：8
相关论文
共 50 条
  • [1] Agrobacterium tumefaciens-mediated transformation of Artemisia annua L and regeneration of transgenic plants
    Vergauwe, A
    Cammaert, R
    Vandenberghe, D
    Genetello, C
    Inze, D
    VanMontagu, M
    VandenEeckhout, E
    [J]. PLANT CELL REPORTS, 1996, 15 (12) : 929 - 933
  • [2] Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua l.
    Vergauwe, A
    Van Geldre, E
    Inzé, D
    Van Montagu, M
    Van den Eeckhout, E
    [J]. PLANT CELL REPORTS, 1998, 18 (1-2) : 105 - 110
  • [3] Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L.
    A. Vergauwe
    E. Van Geldre
    D. Inzé
    M. Van Montagu
    E. Van den Eeckhout
    [J]. Plant Cell Reports, 1998, 18 : 105 - 110
  • [4] Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation
    Chen, DH
    Ye, HC
    Li, GF
    [J]. PLANT SCIENCE, 2000, 155 (02) : 179 - 185
  • [5] Agrobacterium tumefaciens-mediated genetic transformation of Digitalis purpurea L.
    Naivy Pérez-Alonso
    Borys Chong-Pérez
    Alina Capote
    Anabel Pérez
    Yovanny Izquierdo
    Geert Angenon
    Elio Jiménez
    [J]. Plant Biotechnology Reports, 2014, 8 : 387 - 397
  • [6] Agrobacterium tumefaciens-mediated genetic transformation of Digitalis purpurea L.
    Perez-Alonso, Naivy
    Chong-Perez, Borys
    Capote, Alina
    Perez, Anabel
    Izquierdo, Yovanny
    Angenon, Geert
    Jimenez, Elio
    [J]. PLANT BIOTECHNOLOGY REPORTS, 2014, 8 (05) : 387 - 397
  • [7] Agrobacterium tumefaciens-mediated genetic transformation of rye (Secale cereale L.)
    Popelka, JC
    Altpeter, F
    [J]. MOLECULAR BREEDING, 2003, 11 (03) : 203 - 211
  • [8] Agrobacterium tumefaciens-mediated genetic transformation of rye (Secale cereale L.)
    Juan Carlos Popelka
    Fredy Altpeter
    [J]. Molecular Breeding, 2003, 11 : 203 - 211
  • [9] Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)
    Yadav, Manju
    Chaudhary, Darshna
    Sainger, Manish
    Jaiwal, Pawan K.
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2010, 103 (03) : 377 - 386
  • [10] Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)
    Manju Yadav
    Darshna Chaudhary
    Manish Sainger
    Pawan K. Jaiwal
    [J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2010, 103 : 377 - 386
12345