The effects of cell separation with hydrocyclones on the viability of CHO cells

被引：2
|
作者：
Pinto, Rodrigo C. V. [1 ]
Medronho, Ricardo A. [2 ]
Castilho, Leda R. [1 ]
机构：
[1] Univ Fed Rio de Janeiro, COPPE, CxP 68502, BR-21941972 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Sch Chem, Dept Chem, BR-21941972 Rio De Janeiro, Brazil
来源：
CELL TECHNOLOGY FOR CELL PRODUCTS | 2007年
关键词：
hydrocyclone; cell retention device; perfusion; CHO.K1; cells; separation efficiency; cell viability; apoptosis;
D O I：
10.1007/978-1-4020-5476-1_134
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Five different hydrocyclone geometries were tested for the separation of CHO cells and presented separation efficiencies over 97%. Cell passage through the hydrocyclone did not induce apoptosis. The high separation efficiencies coupled to the low viability losses obtained in the present work confirm that hydrocyclones are suitable for animal cell retention in perfusion processes.
引用
收藏
页码：745 / +
页数：2
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