Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells of Phanerochaete chrysosporium in a tubular air-lift bioreactor

被引:12
|
作者
Darah, I [1 ]
Ibrahim, CO [1 ]
机构
[1] Univ Sains Malaysia, Sch Biol Sci, Fermentat & Enzyme Technol Lab, Minden 11800, Penang, Malaysia
关键词
D O I
10.1007/BF02816503
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of lignin-degrading enzymes by free and entrapped cells of Phanerocoete chrysosporium in a tubular air-lift bioreactor was studied. Under optimized cultural conditions the production of lignin peroxidase by free cells, calcium-alginate-entrapped cells and scouring-mesh-entrapped cells was in a ratio of 520:720:950 mU/mL, while the production of manganese peroxidase was in a ratio of 350:480:620 mU/mL. The stability of the entrapped cells by fed-batch systems was highest after 3 feeding experiments which is similarly demonstrated in the repeated use of the preparations in batch system.
引用
收藏
页码:161 / 168
页数:8
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  • [1] Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochœte chrysosporium in a tubular air-lift bioreactor
    I. Darah
    C. O. Ibrahim
    [J]. Folia Microbiologica, 1998, 43 : 161 - 168
  • [2] Lignin-degrading enzyme production by entrapped cells of Phanerochaete chrysosporium in submerged culture system
    Darah, I
    Ibrahim, CO
    [J]. ASIA-PACIFIC JOURNAL OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY, 1997, 5 (03): : 143 - 154
  • [3] The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes
    Singh, Deepak
    Chen, Shulin
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 81 (03) : 399 - 417
  • [4] The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes
    Deepak Singh
    Shulin Chen
    [J]. Applied Microbiology and Biotechnology, 2008, 81 : 399 - 417
  • [5] Effect of agitation on production of lignin-degrading enzymes by Phanerochaete chrysosporium grown in shake-flask cultures.
    Darah, I
    Ibrahim, CO
    [J]. ASIA-PACIFIC JOURNAL OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY, 1996, 4 (03): : 174 - 182
  • [6] Production of lignin-degrading enzymes on solid straw medium by Phanerochaete chrysosporium and Ceriporiopsis subvermispora and degradation of the lignocellulosic substrate
    Hatakka, AI
    Vares, T
    Bocchini, P
    Galletti, GC
    [J]. 1997 BIOLOGICAL SCIENCES SYMPOSIUM, 1997, : 19 - 23