Synthesis of [14C]-L-tryptophan and [14C]-5′-hydroxy-L-tryptophan labeled in the carboxyl group

The synthesis of selectively C-14-labeled L-tryptophan and its derivative 5-hydroxy-L-tryptophan using chemical and multienzymatic methods is reported. The mixture containing [1-C-14[-DL-alanine, indole or 5-hydroxyindole has been converted to [1-C-14]-L-tryptophan or 5'-hydroxy-[1-C-14]-L-tryptophan, respectively, in a one-pot multienzymatic reaction using four enzymes: D-amino acid oxidase, catalase, glutamic-pyruvic transaminase and tryptophanase. Copyright (C) 2003 John Wiley Sons, Ltd.