Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples

被引：10
|
作者：
Inomata, A. [2 ]
Kishida, N. [3 ]
Momoda, T. [4 ]
Akiba, M. [3 ]
Izumiyama, S. [1 ]
Yagita, K. [1 ]
Endo, T. [1 ]
机构：
[1] Natl Inst Infect Dis, Dept Parasitol, Shinjuku Ku, Tokyo 1628640, Japan
[2] Tokyo Metropolitan Inst Publ Hlth, Dept Environm Hlth & Toxicol, Shinjuku Ku, Tokyo 1690073, Japan
[3] Natl Inst Publ Hlth, Dept Water Supply Engn, Wako, Saitama 3510197, Japan
[4] Eiken Chem CO LTD, Biochem Res Lab, Nogi, Tochigi 3290114, Japan
来源：
WATER SCIENCE AND TECHNOLOGY | 2009年 / 60卷 / 08期
关键词：
Cryptosporidiosis; Cryptosporidium; LAMP; oocysts; RT-LAMP; MILWAUKEE; OUTBREAK; PARVUM; DISINFECTION; WISCONSIN; ILLNESS; OOCYSTS; DNA; PCR;
D O I：
10.2166/wst.2009.599
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
We describe a novel assay for simple, rapid and high-sensitive detection of Cryptosporidium oocysts in water samples using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The assay is based on the detection of 18S rRNA specific for Cryptosporidium oocysts. The detection limit of the developed RT-LAMP assay was as low as 6 x 10(-3) oocysts/test tube, which theoretically enables us to detect a Cryptosporidium oocyst and perform duplicated tests even if water samples contain only one oocyst. The developed RT-LAMP assay could more sensitively detect Cryptosporidium oocysts in real water samples than the conventional assay based on microscopic observation.
引用
收藏
页码：2167 / 2172
页数：6
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