Neuropixels 2.0: A miniaturized high-density probe for stable, long-term brain recordings

被引：406

作者：

Steinmetz, Nicholas A. ^{[1
,2
]}

Aydin, Cagatay ^{[3
]}

Lebedeva, Anna ^{[4
]}

Okun, Michael ^{[5
,6
,7
]}

Pachitariu, Marius ^{[8
]}

Bauza, Marius ^{[4
]}

Beau, Maxime ^{[9
]}

Bhagat, Jai ^{[7
]}

Bohm, Claudia ^{[8
]}

Broux, Martijn ^{[3
]}

Chen, Susu ^{[8
]}

Colonell, Jennifer ^{[8
]}

Gardner, Richard J. ^{[10
]}

Karsh, Bill ^{[8
]}

Kloosterman, Fabian ^{[3
,11
,12
,13
]}

Kostadinov, Dimitar ^{[9
]}

Mora-Lopez, Carolina ^{[11
]}

O'Callaghan, John ^{[11
]}

Park, Junchol ^{[8
]}

Putzeys, Jan ^{[11
]}

Sauerbrei, Britton ^{[8
]}

van Daal, Rik J. J. ^{[3
,14
,15
]}

Vollan, Abraham Z. ^{[10
]}

Wang, Shiwei ^{[11
]}

Welkenhuysen, Marleen ^{[11
]}

Ye, Zhiwen ^{[2
]}

Dudman, Joshua T. ^{[8
]}

Dutta, Barundeb ^{[11
]}

Hantman, Adam W. ^{[8
]}

Harris, Kenneth D. ^{[7
]}

Lee, Albert K. ^{[8
]}

Moser, Edvard, I ^{[10
]}

O'Keefe, John ^{[4
]}

Renart, Alfonso ^{[16
]}

Svoboda, Karel ^{[8
]}

Hausser, Michael ^{[9
]}

Haesler, Sebastian ^{[3
,12
]}

Carandini, Matteo ^{[1
]}

Harris, Timothy D. ^{[8
]}

机构：

[1] UCL, UCL Inst Ophthalmol, London, England

[2] Univ Washington, Dept Biol Struct, Seattle, WA 98195 USA

[3] Neuroelect Res Flanders, Leuven, Belgium

[4] UCL, Sainsbury Wellcome Ctr, London, England

[5] Univ Leicester, Ctr Syst Neurosci, Leicester, Leics, England

[6] Univ Leicester, Dept Neurosci Psychol & Behav, Leicester, Leics, England

[7] UCL, UCL Queen Sq Inst Neurol, London, England

[8] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA

[9] UCL, Wolfson Inst Biomed Res, London, England

[10] Norwegian Univ Sci & Technol, Kavli Inst Syst Neurosci, Trondheim, Norway

[11] IMEC, Leuven, Belgium

[12] Vlaams Inst Biotechnol VIB, Leuven, Belgium

[13] Katholieke Univ Leuven, Brain & Cognit, Leuven, Belgium

[14] ATLAS Neuroengn, Leuven, Belgium

[15] Katholieke Univ Leuven, Micro & Nanosyst, Leuven, Belgium

[16] Champalimaud Ctr Unknown, Lisbon, Portugal

来源：

SCIENCE | 2021年 / 372卷 / 6539期

基金：

欧洲研究理事会; 英国生物技术与生命科学研究理事会; 美国国家卫生研究院; 英国惠康基金;

关键词：

NEURONS; ELECTROPHYSIOLOGY; REPRESENTATION; HETEROGENEITY; STABILITY; RESPONSES; MOVEMENT; CORTEX; ARRAY;

D O I：

10.1126/science.abf4588

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Measuring the dynamics of neural processing across time scales requires following the spiking of thousands of individual neurons over milliseconds and months. To address this need, we introduce the Neuropixels 2.0 probe together with newly designed analysis algorithms. The probe has more than 5000 sites and is miniaturized to facilitate chronic implants in small mammals and recording during unrestrained behavior. High-quality recordings over long time scales were reliably obtained in mice and rats in six laboratories. Improved site density and arrangement combined with newly created data processing methods enable automatic post hoc correction for brain movements, allowing recording from the same neurons for more than 2 months. These probes and algorithms enable stable recordings from thousands of sites during free behavior, even in small animals such as mice.

引用

页码：258 / +

页数：43

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