Exercise elevates plasma levels but not gene expression of IL-1β, IL-6, and TNF-α in blood mononuclear cells

被引:164
|
作者
Moldoveanu, AI
Shephard, RJ
Shek, PN
机构
[1] Def & Civil Inst Environm Med, Biomed Sci Sect, Toronto, ON M3M 3B9, Canada
[2] Univ Toronto, Grad Dept Community Hlth, Fac Med, Toronto, ON M5S 2W6, Canada
[3] Univ Toronto, Fac Phys Educ & Hlth, Grad Dept Exercise Sci, Toronto, ON M5S 2W6, Canada
关键词
physical exertion; immune; messenger RNA; peripheral blood;
D O I
10.1152/jappl.2000.89.4.1499
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Physical activity induces a subclinical inflammatory response, mediated in part by leukocytes, and manifested by elevated concentrations of circulating proinflammatory cytokines, including interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha (TNF-alpha). However, the source of the cytokines that appear during exercise remains unknown. In this study, we examined exercise-induced changes in plasma cytokine concentrations and their corresponding mRNA expression in peripheral blood mononuclear cells. Ten healthy [peak oxygen uptake = 48.8 +/- 6.5 (SD) ml. kg(-1).min(-1)] but untrained men [age = 25 +/- 5 (SD) yr] undertook 3 h of exercise (cycling and inclined walking) at 60-65% peak oxygen uptake. Circulating leukocyte subset counts were elevated during and 2 h postexercise but returned to normal within 24 h. Plasma concentrations of IL-1 beta, IL-6, and TNF-alpha peaked at the end of exercise and remained elevated at 2 h (IL-6) and up to 24 h (IL-1 beta and TNF-alpha) postexercise. Cytokine gene expression in circulating mononuclear cells was measured by using the reverse transcriptase-polymerase chain reaction; mRNA accumulation did not change with exercise. In conclusion, mRNA accumulation of IL-1 beta, IL-6, and TNF-alpha in circulating mononuclear cells is not affected by 3 h of moderate endurance exercise and does not seem to account for the observed increases in plasma cytokines.
引用
收藏
页码:1499 / 1504
页数:6
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