Purification of a heat-stable activator protein for ADP-ribosylation factor-dependent phospholipase D

被引：4
|
作者：
Akisue, T [1 ]
Jinnai, H [1 ]
Hitomi, T [1 ]
Miwa, N [1 ]
Yoshida, K [1 ]
Nakamura, S [1 ]
机构：
[1] Kobe Univ, Sch Med, Dept Biochem, Kobe, Hyogo 650, Japan
来源：
FEBS LETTERS | 1998年 / 422卷 / 01期
关键词：
phospholipase D; ADP-ribosylation factor; phospholipase D activator; phosphatidylcholine; phosphatidylethanolamine;
D O I：
10.1016/S0014-5793(97)01611-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A heat-stable activator for ADP-ribosylation factor (ARF)-dependent phospholipase D (PLD) was purified to near homogeneity from rat kidney cytosol by a sequential column chromatography. The purified activator has a molecular mass of 23 kDa on SDS-PAGE. Using a partially purified ARF-dependent PLD from rat kidney, the activator synergistically stimulates PLD with ARF in time- and dose-dependent manner, In the absence of ARF, the activator has little or no effect, The purified activator also stimulates PLD under several conditions including permeabilized cell system, suggesting that the activator is a physiologically relevant regulator of PLD. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：108 / 112
页数：5
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