Frequency, Antimicrobial Resistance and Genetic Diversity of Klebsiella pneumoniae in Food Samples

被引:51
|
作者
Guo, Yumei [1 ]
Zhou, Haijian [2 ,3 ]
Qin, Liyun [1 ]
Pang, Zhizhao [1 ]
Qin, Tian [2 ,3 ]
Ren, Hongyu [2 ]
Pan, Zhuo [1 ]
Zhou, Jikun [1 ]
机构
[1] Shijiazhuang Ctr Dis Control & Prevent, Shijiazhuang, Peoples R China
[2] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China
[3] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China
来源
PLOS ONE | 2016年 / 11卷 / 04期
关键词
SPECTRUM-BETA-LACTAMASE; MEDIATED QUINOLONE RESISTANCE; EXTENDED-SPECTRUM; FLUOROQUINOLONE RESISTANCE; MOLECULAR CHARACTERIZATION; CARBAPENEM RESISTANCE; ESCHERICHIA-COLI; CHICKEN MEAT; PREVALENCE; GYRA;
D O I
10.1371/journal.pone.0153561
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
This study aimed to assess the frequency of Klebsiella pneumoniae in food samples and to detect antibiotic resistance phenotypes, antimicrobial resistance genes and the molecular subtypes of the recovered isolates. A total of 998 food samples were collected, and 99 (9.9%) K. pneumoniae strains were isolated; the frequencies were 8.2% (4/49) in fresh raw seafood, 13.8% (26/188) in fresh raw chicken, 11.4% (34/297) in frozen raw food and 7.5% (35/464) in cooked food samples. Antimicrobial resistance was observed against 16 antimicrobials. The highest resistance rate was observed for ampicillin (92.3%), followed by tetracycline (31.3%), trimethoprim-sulfamethoxazole (18.2%), and chloramphenicol (10.1%). Two K. pneumoniae strains were identified as extended-spectrum beta-lactamase (ESBL)-one strain had three beta-lactamases genes (bla(SHV), bla(CTX-M-1), and bla(CTX-M-10)) and one had only the bla(SHV) gene. Nineteen multidrug-resistant (MDR) strains were detected; the percentage of MDR strains in fresh raw chicken samples was significantly higher than in other sample types (P<0.05). Six of the 18 trimethoprim-sulfamethoxazole-resistant strains carried the folate pathway inhibitor gene (dhfr). Four isolates were screened by PCR for quinolone resistance genes; aac(6')-Ib-cr, qnrB, qnrA and qnrS were detected. In addition, gyrA gene mutations such as T247A (Ser83Ile), C248T (Ser83Phe), and A260C (Asp87Ala) and a parC C240T (Ser80Ile) mutation were identified. Five isolates were screened for aminoglycosides resistance genes; aacA4, aacC2, and aadA1 were detected. Pulsed-field gel electrophoresis-based subtyping identified 91 different patterns. Our results indicate that food, especially fresh raw chicken, is a reservoir of antimicrobial-resistant K. pneumoniae, and the potential health risks posed by such strains should not be underestimated. Our results demonstrated high prevalence, antibiotic resistance rate and genetic diversity of K. pneumoniae in food in China. Improved control and prevention strategies are urgently needed.
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