Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method

A simple fast, accurate, precise and cost effective reverse phase high performance liquid chromatographic method is developed for simultaneous determination of halobetasol propionate and salicylic acid impurities in the ointment formulation The chromatography equipped with inertsil C-8 (250 mm x 46 mm) column using a mobile phase of buffer (0 1% of orthophosphoric acid) and acetontrile with a flow rate of 1 5 mL/min, with ultraviolet detector configured at 231 nm The method is found to be linear in the range of 0.118- 17 734 mu g/mL for 4-hydroxy benzoic acid. 0 120-9 036 mu g/mL for 4-hydroxy isophthalic acid, 0.12-3 586 mu g/mL for phenol, 0.103-8.987 mu g/mL for salicylic acid. 0 069-1 480 mu g/mL for diflorasone 21 propionate. 0.071-1.472 mu g/mL for diflorasone 17 propionate 21 mesylate and 0 057-1 514 mu g/mL for halobetasol propionate.