Derivation of primitive neural stem cells from human-induced pluripotent stem cells

被引:3
|
作者
Shin, Woo Jung [1 ]
Seo, Ji-Hye [2 ,3 ]
Choi, Hyun Woo [4 ]
Hong, Yean Ju [1 ]
Lee, Won Ji [1 ]
Chae, Jung Il [2 ,3 ]
Kim, Sung Joo [5 ,6 ]
Lee, Jeong Woong [7 ]
Hong, Kwonho [1 ]
Song, Hyuk [1 ]
Park, Chankyu [1 ]
Do, Jeong Tae [1 ]
机构
[1] Konkuk Univ, KU Inst Sci & Technol, Dept Stem Cell & Regenerat Biotechnol, 120 Neungdong Ro, Seoul 05029, South Korea
[2] Chonbuk Natl Univ, Dept Dent Pharmacol, Sch Dent, Jeonju, South Korea
[3] Chonbuk Natl Univ, BK21 Plus, Inst Oral Biosci, Jeonju, South Korea
[4] Chonbuk Natl Univ, Dept Anim Sci, Coll Agr Life Sci, Jeonju, South Korea
[5] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Mol Med, Seoul, South Korea
[6] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Surg, Seoul, South Korea
[7] Korea Res Inst Biosci & Biotechnol, Res Ctr Integrat Cellul, Daejeon, South Korea
基金
新加坡国家研究基金会;
关键词
differentiation; human-induced pluripotent stem cells; neural lineage; primitive neural stem cells; HUMAN ES CELLS; IPS CELLS; EFFICIENT; DIFFERENTIATION; EXPRESSION; NEURONS; TUMORIGENICITY; INTEGRATION; INHIBITION; GENERATION;
D O I
10.1002/cne.24727
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Human-induced pluripotent stem cells (hiPSCs) have facilitated studies on organ development and differentiation into specific lineages in in vitro systems. Although numerous studies have focused on cellular differentiation into neural lineage using hPSCs, most studies have initially evaluated embryoid body (EB) formation, eventually yielding terminally differentiated neurons with limited proliferation potential. This study aimed to establish human primitive neural stem cells (pNSCs) from exogene-free hiPSCs without EB formation. To derive pNSCs, we optimized N2B27 neural differentiation medium through supplementation of two inhibitors, CHIR99021 (GSK-3 inhibitor) and PD0325901 (MEK inhibitor), and growth factors including basic fibroblast growth factor (bFGF) and human leukemia inhibitory factor (hLIF). Consequently, pNSCs were efficiently derived and cultured over a long term. pNSCs displayed differentiation potential into neurons, astrocytes, and oligodendrocytes. These early NSC types potentially promote the clinical application of hiPSCs to cure human neurological disorders.
引用
收藏
页码:3023 / 3033
页数:11
相关论文
共 50 条
  • [1] Derivation of neural crest cells from human pluripotent stem cells
    Lee, Gabsang
    Chambers, Stuart M.
    Tomishima, Mark J.
    Studer, Lorenz
    [J]. NATURE PROTOCOLS, 2010, 5 (04) : 688 - 701
  • [2] Derivation of neural crest cells from human pluripotent stem cells
    Gabsang Lee
    Stuart M Chambers
    Mark J Tomishima
    Lorenz Studer
    [J]. Nature Protocols, 2010, 5 : 688 - 701
  • [3] Efficient and Rapid Derivation of Primitive Neural Stem Cells and Generation of Brain Subtype Neurons From Human Pluripotent Stem Cells
    Yan, Yiping
    Shin, Soojung
    Jha, Balendu Shekhar
    Liu, Qiuyue
    Sheng, Jianting
    Li, Fuhai
    Zhan, Ming
    Davis, Janine
    Bharti, Kapil
    Zeng, Xianmin
    Rao, Mahendra
    Malik, Nasir
    Vemuri, Mohan C.
    [J]. STEM CELLS TRANSLATIONAL MEDICINE, 2013, 2 (11) : 862 - 870
  • [4] Generation of human-induced pluripotent stem cells
    Park, In-Hyun
    Lerou, Paul H.
    Zhao, Rui
    Huo, Hongguang
    Daley, George Q.
    [J]. NATURE PROTOCOLS, 2008, 3 (07) : 1180 - 1186
  • [5] Generation of human-induced pluripotent stem cells
    In-Hyun Park
    Paul H Lerou
    Rui Zhao
    Hongguang Huo
    George Q Daley
    [J]. Nature Protocols, 2008, 3 : 1180 - 1186
  • [6] Requirements for human-induced pluripotent stem cells
    Zhang, Ying
    Wei, Jun
    Cao, Jiani
    Zhang, Kehua
    Peng, Yaojin
    Deng, Hongkui
    Kang, Jiuhong
    Pan, Guangjin
    Zhang, Yong
    Fu, Boqiang
    Hu, Shijun
    Na, Jie
    Liu, Yan
    Wang, Lei
    Liang, Lingmin
    Zhu, Huanxin
    Zhang, Yu
    Jin, Zi-Bing
    Hao, Jie
    Ma, Aijin
    Zhao, Tongbiao
    Yu, Junying
    [J]. CELL PROLIFERATION, 2022, 55 (04)
  • [7] Inhibition of Notch signaling facilitates the differentiation of human-induced pluripotent stem cells into neural stem cells
    Chun-Yuan Chen
    Wei Liao
    Yuan-Lei Lou
    Qing Li
    Bin Hu
    Yang Wang
    Zhi-Feng Deng
    [J]. Molecular and Cellular Biochemistry, 2014, 395 : 291 - 298
  • [8] Inhibition of Notch signaling facilitates the differentiation of human-induced pluripotent stem cells into neural stem cells
    Chen, Chun-Yuan
    Liao, Wei
    Lou, Yuan-Lei
    Li, Qing
    Hu, Bin
    Wang, Yang
    Deng, Zhi-Feng
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2014, 395 (1-2) : 291 - 298
  • [9] The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells
    Munoz, Javier
    Low, Teck Y.
    Kok, Yee J.
    Chin, Angela
    Frese, Christian K.
    Ding, Vanessa
    Choo, Andre
    Heck, Albert J. R.
    [J]. MOLECULAR SYSTEMS BIOLOGY, 2011, 7
  • [10] Comparison of the glycosphingolipids of human-induced pluripotent stem cells and human embryonic stem cells
    Saljo, Karin
    Barone, Angela
    Vizlin-Hodzic, Dzeneta
    Johansson, Bengt R.
    Breimer, Michael E.
    Funa, Keiko
    Teneberg, Susann
    [J]. GLYCOBIOLOGY, 2017, 27 (04) : 291 - 305