Characterization of polymorphic microsatellites in the Pacific sardine Sardinops sagax sagax (Clupeidae)

被引：9
|
作者：
Pereyra, RT
Saillant, E
Pruett, CL
Rexroad, CE
Rocha-Olivares, A
Gold, AR
机构：
[1] CICESE, Dept Oceanog Biol, Ensenada 22860, Baja California, Mexico
[2] Texas A&M Univ, Ctr Biosystemat & Biodivers, College Stn, TX 77843 USA
[3] USDA ARS, Natl Ctr Cold & Cool Water Aquaculture, Kearneysville, WV 25430 USA
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期
关键词：
genomic library; marine fish; microsatellites; Pacific sardine; PCR primers;
D O I：
10.1111/j.1471-8286.2004.00801.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We isolated 11 microsatellites from the Pacific sardine Sardinops sagax sagax. The number of alleles and H-E among 30 individuals from a single population ranged from four to 24 and from 0.606 to 0.959, respectively. Pacific sardines are a vital economic resource in the northeastern Pacific Ocean, but insufficiently polymorphic loci have limited inferences about its stock structure and genetic variation. The level of variability of these new markers will prove useful in testing hypotheses on the stock-structure and long-term genetic integrity of the species.
引用
收藏
页码：739 / 741
页数：3
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