In vitro antidiabetic activities and GC-MS phytochemical analysis of Ximenia americana extracts

Objective: To ascertain various phytochemical ingredients in Ximenia americana leaves extracts by GC-MS & evaluating their antidiabetic property by using in vitro assays. Methods: The serial extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were subjected to GC-MS analysis and also the antidiabetic activity was assessed by employing standard in vitro techniques. Results: GC-MS analysis confirmed the presence of different phytochemicals in each leaf extracts of X. americana. The major phytoconstituents were found to be Oleic acid, n-Hexadecanoic acid, Non-decanoic acid and Octadecatrienoic acid in chloroform extract; and 3-Undecene, Tridecene, Trifluoroaceteoxy tetradecene, and Trichloroacetic acid-3-tridecyl-ester in ethyl acetate extract; where in 1-Tetracosanol, Behenyl alcohol, 1-Hexacosanol, Octadecanal, 4-Piperidine propanoic acid and alpha-D-mannofuranoside in methanol extract; 8,11,14-Eicosatrienoic acid, 7-Tetradecanal and 1-Ocytyn-3-Ol-4-Ethy in ethanol extract; aqueous extract showed the presence of 9,12-Octadecandionoic acid. In vitro antidiabetic studies show that aqueous extract exhibited significant activity when compared to other solvent extracts. Conclusion: The investigation confirms that aqueous extract exhibited highest antidiabetic activity among all extracts; Additional studies on needed for purification, characterization and structural elucidation of bioactive compounds from aqueous extract & also confirm its antidiabetic property by in vivo studies. This study provides scientific evidence that leaves of Ximenia americana have anti-diabetic efficacy. Thus, considering its relative hypoglycemic potency, they may serve as useful therapeutic agents for treating diabetes. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.