Use of Hydrogenophaga pseudoflava Penetration To Quantitatively Assess the Impact of Filtration Parameters for 0.2-Micrometer-Pore-Size Filters

被引：9

作者：

Lee, A. ^{[1
]}

McVey, J. ^{[2
]}

Faustino, P. ^{[4
]}

Lute, S. ^{[1
]}

Sweeney, N. ^{[3
]}

Pawar, V. ^{[2
]}

Khan, M. ^{[4
]}

Brorson, K. ^{[1
]}

Hussong, D. ^{[2
]}

机构：

[1] US FDA, Off Biotechnol Prod, Silver Spring, MD 20903 USA

[2] US FDA, Off Pharmaceut Sci New Drug Microbiol Staff, Silver Spring, MD 20903 USA

[3] US FDA, Off Gener Drugs, Silver Spring, MD 20903 USA

[4] US FDA, Off Testing & Res, Silver Spring, MD 20903 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 03期

关键词：

MICROBIAL REMOVAL PERFORMANCE; BACTERIAL CHALLENGE TESTS; WATER-BORNE BACTERIA; RATED FILTERS; ESCHERICHIA-COLI; CELL-SIZE; PART II; STERILE FILTRATION; MEMBRANE FILTERS; RETENTION;

D O I：

10.1128/AEM.01825-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Filters rated as having a 0.2-mu m pore size (0.2-mu m-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. Using Hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-mu m-rated filters and the impact of filtration process parameters and bacterial challenge density. We show that consistent H. pseudoflava passage occurs through 0.2-mu m-rated filters. This is in contrast to an absence of significant passage of nutritionally challenged bacteria that are of similar size (i.e., hydrodynamic diameter) but dissimilar geometry.

引用

页码：695 / 700

页数：6