Analysis of fructose, glycine and triglycine using HPLC UV/vis detection and evaporate light-scattering detection

The development of a method for the simultaneous determination of glycine, triglycine and fructose using UV/vis and evaporative light-scattering detection (ELSD) is described. This was necessary as part of a research project dealing with the recovery of functional peptides from aqueous streams on an industrial scale using adsorption or related technologies. Fructose is barely detectable by UV/vis as it lacks detectable functionalities, while glycine and triglycine are both UV/vis sensitive. An NH2 phase was chosen as a column and separation was obtained within seven minutes on a 250 x 4.6 mm column. Limits of detection are approximately 40 mg fructose/L, 4 mg glycine/L and 0.05 mg triglycine/L. Calibration functions are linear in a range of 40-1400 mg/L for fructose, 5-200 mg/L for glycine and 0.5-70 mg/L for triglycine.