Evaluation of 99mTc-sulfadiazine as Bacillus microorganisms infection imaging agent using animal model

Bacterial infection is one of the vital sources of morbidity and mortality. The development of single photon emission computed tomography (SPECT) radiotracer agents using antibiotics, for targeting in-vivo bacteria, helps in antibiotic dose calibration, targeted infection therapy and reduction in mortality rate. The aim of this study was to appraised Tc-99m-labeling sulfadiazine as a radiopharmaceutical for bacillus infections imaging. Radiolabeling of sulfadiazine with technetium-99m was carried out by subsequent addition of 1.5 mL aqueous solution of sulfadiazine (1mg/mL), 120 mu g stannous tartrate, gentistic acid as stabilizing agent and 185 MBq normal saline solution of (TcO4-1)-Tc-99m (pertechnetate) at pH = 5. The reaction mixture was incubated for 40 min at room temperature with light stirring. The quality control analysis (ITLC-SG and paper chromatography analysis) revealed similar to 98% labeling yield. Biodistribution and scintigraphic study was carried using bacillus bacterial infection induced New Zealand white rabbits. Due to the ease of Tc-99m-sulfadiazine conjugation method, high labeling efficiency, shelf stability (> 95% up to 6h), blood serum stability (similar to 90% up to 6h) and high uptake in the infected muscle (T/NT =2.21 at 1h), Tc-99m-SDZ could be used as radiopharmaceutical of choice for further pre-clinical and clinical studies.