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Quantitative counting of Bifidobacterium spp. in a sample mixed with Lactobacillus acidophilus

被引：0
|
作者：
Park, YM [1 ]
So, JS [1 ]
机构：
[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 1998年 / 8卷 / 02期
关键词：
Bifidobacterium; quantitation; PCR;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
PCR was used for quantitative counting of Bifidobacterium spp. in a sample mixed with Lactobacillus acidophilus using two primer sets; one set for universal priming and the other set for Bifidobacterium specific priming. DNA products from two independent PCRs with DNA extracted from the mixed sample were found to be easily distinguishable from each other by agarose gel electrophoresis. The concentrations of PCR products correlated with the total number of bacteria and with the number of Bifidobacterium spp. present in the sample.
引用
收藏
页码：182 / 184
页数：3
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