Translation efficiency mediated by the 5′ untranslated region greatly affects protein production in Aspergillus oryzae

被引：31
|
作者：
Koda, A [1 ]
Minetoki, T [1 ]
Ozeki, K [1 ]
Hirotsune, M [1 ]
机构：
[1] Ozeki Corp, Gen Res Lab, Nishinomiya, Hyogo 6638227, Japan
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2004年 / 66卷 / 03期
关键词：
D O I：
10.1007/s00253-004-1681-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We demonstrate that the 5' untranslated region ( 5' UTR) plays an important role in determining translation efficiency in Aspergillus oryzae, using a model beta-glucuronidase (GUS) expression system. Alterations in the 5' UTR resulted in an increase in GUS activity of up to eightfold, without affecting mRNA levels. Moreover, using the most effective 5' UTR construct, we could achieve remarkable intracellular overproduction of GUS protein; and the GUS level reached more than 50% of the total soluble protein. This is the first experimental evidence indicating the feasibility of improving recombinant protein yield by promoting translation initiation in filamentous fungi.
引用
收藏
页码：291 / 296
页数：6
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