Enantiomeric separation using erythromycin as a new capillary zone electrophoresis chiral selector

The chiral selectivity of erythromycin was examined in capillary zone electrophoresis using a fused silica capillary column. Excellent chiral separations of four derivatives of biphenyldimethylester for antihepatitis drugs (A1-A4) have been achieved, respectively. The effects of various experimental factors such as chiral selector concentration and pH value, voltage and temperature, organic modifiers on enantiomeric resolution were investigated. Enatioresolution was found to significantly improve with increasing chiral selector concentration while the migration time of the analytes showed slight increase. Enantioselectivities of the chiral solutes were considerable influenced by the content of organic modifieres in running buffer. For chiral separation of the above racemates, the optimal run-buffer consisting of 20 mM erythromycin, 50 mM phosphate and 50% methanol (pH = 6) was obtained by experiment.