A red-emission fluorescence probe based on 1,4-addition reaction mechanism for the detection of biothiols in vitro and in vivo

被引：0
|
作者：
Hu, Yaoyun [1 ]
Shang, Zhuye [1 ]
Gu, Pengli [2 ]
He, Guangjie [2 ]
Zhang, Run [3 ]
Meng, Qingtao [1 ]
Zhang, Zhiqiang [1 ]
机构：
[1] Univ Sci & Technol Liaoning, Sch Chem Engn, Anshan 114051, Liaoning, Peoples R China
[2] Xinxiang Med Univ, Sch Forens Med, Jinsui Rd 601, Xinxiang 453003, Henan, Peoples R China
[3] Univ Queensland, Australian Inst Bioengn & Nanotechnol, Brisbane, Qld 4072, Australia
来源：
ANALYTICAL SCIENCES | 2022年 / 38卷 / 03期
关键词：
Fluorescence probe; Biothiols; Nucleophilic addition; Bioimaging; NEAR-INFRARED FLUORESCENCE; SELECTIVE DETECTION; GLUTATHIONE; CYSTEINE; CYS; GSH; CELLS;
D O I：
10.2116/analsci.21P177
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this work, a new fluorescence probe (DC) with a donor-pi-acceptor (D-pi-A) structure was designed and synthesized for the detection of three kinds of biothiols (Cys, Hcy and GSH) in live cells and organisms. DC displayed an intense red-emission centered at 625 nm. In the presence of biothiols, the nucleophilic addition reaction between the C=C double bond of DC and the sulfhydryl group (-SH) of biothiols occurred, resulting in obvious fluorescence quenching responses. DC exhibited high selectivity towards biothiols over other common bioactive species with low detection limits (0.26, 0.43, and 0.44 mu M for Cys, Hcy and GSH, respectively). In addition, DC displayed a rapid response to biothiols within 4 min. The applications of DC in biothiols detection and imaging were then successfully demonstrated for the real-time monitoring of endogenous and exogenous biothiols in live cells and live animals.
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收藏
页码：505 / 514
页数：10
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