Linkage among the combined temperature?retention time condition, microbial interaction, community structure, and process performance in the hydrolysis of waste activated sludge

Numerous studies have revealed the effect of temperature and hydraulic retention time (HRT) on microbiota in sludge biological hydrolysis (BH). However, few scholars have explored the combined effect of these two critical BH parameters. This study explored the BH performance and community structures over 12 combined temperatures?HRT conditions for temperatures from 35 ?C to 55 ?C and HRTs from 1.5 days to 6.0 days. Results showed that the 12 combined conditions formed only six distinct community structures with each of them relating to a distinctive range of volatile suspended solid reduction rates. The nonmetric multidimensional scaling and species?species association analysis on the DNA sequencing data revealed that the community structure was greatly driven by the microbial interactions (e.g., heterogeneous commensalism and competition) under the effect of temperature and HRT. This study established the linkages among the combined BH temperature?HRT conditions, microbial interaction, microbial community, and BH performance.