Repeated fed-batch process for improving lovastatin production

被引：75

作者：

Kumar, MS ^{[1
]}

Jana, SK ^{[1
]}

Senthil, V ^{[1
]}

Shashanka, V ^{[1
]}

Kumar, SV ^{[1
]}

Sadhukhan, AK ^{[1
]}

机构：

[1] Dr Reddys Res Fdn, Biotechnol R&D, Fermentat Lab, Hyderabad 500050, Andhra Pradesh, India

来源：

PROCESS BIOCHEMISTRY | 2000年 / 36卷 / 04期

关键词：

lovastatin; fed-batch process; oxygen transfer; submerged cultivation; volumetric productivity; Aspergillus terreus;

D O I：

10.1016/S0032-9592(00)00222-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Submerged cultivation of a high yielding strain of Aspergillus terreus DRCC 122 for the production of lovastatin in the batch process had limited success with a maximum titre of 1270 mg l(-1) in 288 h and an overall volumetric productivity of 4.41 mg l(-1) h(-1) in a 10001 bioreactor. A cost effective repeated fed-batch process with maltodextrin and corn steep liquor feed as carbon and nitrogen sources, respectively, showed a significant increase in lovastatin yield. The final titre was 2200 mg l(-1) in 288 h of fermentation, with overall volumetric productivity of 7.64 mg l(-1) h(-1), showing an increase of 73% over the batch process. The kinetic parameters were studied; maximum specific oxygen uptake rate (Q(O2)) and volumetric mass transfer coefficient (K(L)a) were 0.24 m mole O-2 g(-1) dry cell wt. h(-1) and 280 h(-1), respectively, in fed-batch process. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：363 / 368

页数：6

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