CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES BIOSYNTHESIZED USING (INDIGOFERA OBLONGIFOLIA) LEAVES EXTRACT

Here, we describe the novel biosynthesis of silver nanoparticles (AgNPs) using the extract of Indigofera oblongifolia (Hasr) leaves. The synthesized AgNPs were confirmed and characterized using ultraviolet visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). An extreme absorption was detected at a wavelength near 400 nm after the formation of AgNPs. FTIR determined the extract biomolecules responsible for the reduction of the synthesized AgNPs. An EDS signal at 3 keV confirmed the presence of the Ag signal using surface plasmon resonance. SEM and TEM revealed the generally circular shape of AgNPs with sizes ranging from 8 nm to 25 nm (average particle size 15 nm). The antibacterial activity of AgNPs was pronounced for all bacteria studied.