The influence of inhibition of acid phosphatase, β-N-acetylglucosaminidase and lactate dehydrogenase present in the sperm of ide (Leuciscus idus) on the percentage of fertilised eggs

被引：3

作者：

Sarosiek, Beata ^{[1
]}

Dryl, Katarzyna ^{[1
]}

Palinska-Zarska, Katarzyna ^{[2
]}

Zarski, Daniel ^{[1
]}

机构：

[1] Polish Acad Sci, Dept Gamete & Embryo Biol, Inst Anim Reprod & Food Res, Tuwima Str 10, PL-10748 Olsztyn, Poland

[2] Univ Warmia & Mazury, Dept Ichthyol, Fac Environm Sci, Olsztyn, Poland

来源：

ANIMAL REPRODUCTION SCIENCE | 2018年 / 195卷

关键词：

Ide; Fertilisation; Acid phosphatase; beta-N-acetylglucosaminidase; Lactate dehydrogenase; Motility; TROUT ONCORHYNCHUS-MYKISS; SEMINAL PLASMA; RAINBOW-TROUT; ZONA-PELLUCIDA; SPERMATOZOA; GOSSYPOL; MOTILITY; ARYLSULFATASE; PENETRATE; QUALITY;

D O I：

10.1016/j.anireprosci.2018.05.011

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study investigated how the inhibition of certain enzymes present in ide sperm influences sperm motility and the percentage of fertilised eggs. The enzymes studied were acid phosphatase (AcP), beta-N-acetylglucosaminidase (beta-NAGase) and lactate dehydrogenase (LDH). None of the inhibitors affected ide sperm motility parameters. The addition of gossypol (a LDH inhibitor) caused a considerable increase in the percentage of fertilised eggs (92-95% compared to 63% in the control). The inhibition of AcP caused a considerable decrease in fertility rate at the highest inhibitor dose, the percentage of fertilised eggs decreased to 26%. A similar effect was seen after the addition of acetarnide (a beta-NAGase inhibitor), but in this case the highest dose caused complete inhibition of fertilisation. The results presented here indicate the importance of AcP and beta-NAGase in the process of ide fertilisation.

引用

页码：96 / 101

页数：6