Structure of the O-Antigen and Characterization of the O-Antigen Gene Cluster of Escherichia coli O108 Containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (8-Epilegionaminic) Acid

被引：15
|
作者：
Perepelov, A. V. [1 ]
Liu, Bin [2 ,3 ]
Senchenkova, S. N. [1 ]
Shashkov, A. S. [1 ]
Shevelev, S. D. [1 ]
Feng, Lu [2 ,3 ]
Wang, Lei [2 ,3 ]
Knirel, Y. A. [1 ]
机构：
[1] Russian Acad Sci, Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China
[3] Nankai Univ, Tianjin Key Lab Microbial Funct Genom, TEDA Coll, TEDA, Tianjin 300457, Peoples R China
来源：
BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 01期
基金：
俄罗斯基础研究基金会; 中国国家自然科学基金;
关键词：
Escherichia coli; lipopolysaccharide; O-antigen; biosynthesis; O-polysaccharide structure; O-antigen gene cluster; nonulosonic acid; PSEUDAMINIC ACID; PSEUDOMONAS-AERUGINOSA; BIOSYNTHESIS; POLYSACCHARIDE; IDENTIFICATION; CAMPYLOBACTER; HELICOBACTER; CONVERSION; PATHWAYS; SEQUENCE;
D O I：
10.1134/S0006297910010037
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
On mild acid degradation of the lipopolysaccharide of Escherichia coli O108, the O-polysaccharide was isolated and studied by sugar analysis and one- and two-dimensional H-1- and C-13-NMR spectroscopy. The polysaccharide was found to contain an unusual higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid (di-N-acetyl-8-epilegionaminic acid, 8eLeg5Ac7Ac). The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: -> 4)-alpha-8eLegp5Ac7Ac-(2 -> 6)-alpha-D-Galp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-alpha-D-GlcpNAc-(1 ->. Functions of the E. coli O108 antigen biosynthetic genes, including seven putative genes for synthesis of 8eLeg5Ac7Ac, were assigned by sequencing the O-antigen gene cluster along with comparison with gene databases and known biosynthetic pathways for related nonulosonic acids.
引用
收藏
页码：19 / 24
页数：6
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