Applications of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones

被引：14

作者：

Osamura, RY ^{[1
]}

Itoh, Y

Matsuno, A

机构：

[1] Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan

[2] Teikyo Univ, Ichihara Hosp, Dept Neurosurg, Kanagawa, Japan

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2000年 / 48卷 / 07期

关键词：

immunohistochemistry; in situ hybridization; electron microscopy; plastic embedding; peptide hormones; secretory pathway; secretory granules; messenger RNA; regulated pathway; constitutive pathway;

D O I：

10.1177/002215540004800701

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen-antigen or mRNA-antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells.

引用

页码：885 / 891

页数：7

共 5 条

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1600, Eaton Publishing Co., Natick, MA, USA (02):
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Lian, Jianshe
Jiang, Qing
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JOURNAL OF APPLIED PHYSICS, 2014, 116 (10)
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ACTA HISTOCHEMICA ET CYTOCHEMICA, 1995, 28 (02) : 187 - 189
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ENDOCRINOLOGY, 1994, 135 (03) : 818 - 825

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