The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia

被引：14
|
作者：
Secondo, Agnese [1 ]
Staiano, Ilaria Rosaria [1 ]
Scorziello, Antonella [1 ]
Sirabella, Rossana [1 ]
Boscia, Francesca [1 ]
Adornetto, Annagrazia [1 ]
Canzoniero, Lorella Maria Teresa [1 ]
Di Renzo, Gianfranco [1 ]
Annunziato, Lucio [1 ]
机构：
[1] Univ Naples Federico II, Sch Med, Dept Neurosci, Div Pharmacol, I-80131 Naples, Italy
来源：
SODIUM-CALCIUM EXCHANGE AND THE PLASMA MEMBRANE CA2+-ATPASE IN CELL FUNCTION: FIFTH INTERNATIONAL CONFERENCE | 2007年 / 1099卷
关键词：
Na+/Ca2+ exchanger; chemical hypoxia; neuroprotection; Ca2+](i) homeostasis;
D O I：
10.1196/annals.1387.052
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+](i) increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.
引用
收藏
页码：481 / 485
页数：5
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