A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen

被引：9

作者：

Yang, Junxing ^{[1
,2
]}

Hua, Qunyi ^{[1
]}

Chen, Huanchun ^{[2
]}

Lv, Jianqiang ^{[1
]}

Chen, Bing ^{[1
]}

Ruan, Zhouxi ^{[1
]}

机构：

[1] Shenzhen Entry Exit Inspect & Quarantine Bur, Tech Ctr Anim & Plant Inspect & Quarantine, Shengzhen, Peoples R China

[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Unit Anim Infect Dis, Wuhan, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2010年 / 22卷 / 02期

基金：

中国博士后科学基金;

关键词：

Bluetongue virus; latex agglutination; recombinant VP7 antigen; MONOCLONAL-ANTIBODY; BLOCKING ELISA; SERA;

D O I：

10.1177/104063871002200212

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA). the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.

引用

页码：242 / 244

页数：3

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