QUANTITATIVE AND QUALITATIVE ANALYSIS OF (-) MAMMEA A/BB COUMARIN IN EXTRACTS OF CALOPHYLLUM BRASILIENSE CAMBESS (CLUSIACEAE) BY HPLC

A simple, rapid, and specific high performance liquid chromatographic (HPLC) method was established for analysis of the bioactive compound (-) mammea A/BB in C. brasiliense leaves. In the present study, a method was developed and validated for quantitative determination of (-) mammea A/BB in extracts of C. brasiliense Cambess (Clusiaceae). The analyses were carried out on a Metasil ODS column at 30 degrees C by gradient elution using different concentrations of acetonitrile-water as the mobile phase, flow rate of 0.6mL/min and detection wavelength of 280nm. The validation using (-) mammea A/BB as the standard demonstrated that the method shows linearity (linear correlation coefficient=0.9989), precision (relative standard deviation 1.35%) and accuracy (mean recovery=88.6%) in the concentration range 15.56-250.0 mu g/mL. The limit of detection (LOD) and limit of quantification (LOQ) were 7.61 and 25.36 mu g/mL, respectively. This method allowed the identification and quantification of (-) mammea A/BB in extracts obtained from the leaves, stems, and fruits by maceration. The extracts showed different chromatographic profiles: the leaves presented the highest concentration of (-) mammea A/BB, and the dichloromethane extract of leaves showed a larger quantity of this compound than did the hydroethanolic extract. This HPLC method is suitable for routine quantitative analysis of (-) mammea A/BB in extracts of C. brasiliense and phytopharmaceuticals containing this herb.