Aquaspirillum soli sp nov., isolated from a soil sample

A Gram-stain-negative, smooth, opaque, white-pigmented, helical-shaped, catalase-and oxidase-positive bacterium that was motile by means of bipolar tufts of flagella and grew under microaerophilic conditions, was isolated from a soil sample of a reed pond in Shangqiu, Henan province, PR China. The strain, designated THG-SQE6(T), grows well at 25-42 degrees C, pH 6.5-7.5 and in the presence of 0-1% (w/v) NaCl. hylogenetic analysis based on 16S rRNA gene sequences showed that strain THG-SQE6(T) was most closely related to Aquaspirillum serpens IAM 13944(T) (97.23% 16S rRNA gene sequence similarity). The DNA G+C content of strain THG-SQE6(T) was 53.10 mol%. In DNA-DNA hybridization experiments, the DNA-DNA relatedness between strain THG-SQE6(T) and its closest phylogenetic neighbour was below 62.6 %. The predominant isoprenoid quinone detected in strain THG-SQE6(T) was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. The major fatty acids were identified as C-12 : 0 3-OH, C-16 : 0, C-18 : 0, summed feature 3 and summed feature 8. These data support the affiliation of strain THG-SQE6(T) to the genus Aquaspirillum. Based on findings from the phenotypic, genotypic and phylogenetic characterization of strain THG-SQE6(T), a novel species of the genus Aquaspirillum named Aquaspirillum soli sp. nov. is proposed. The type strain is THG-SQE6 T (= KACC 18846 T = CCTCC AB 2016081 T).