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The effects of amiloride, a Na+-H+ exchange inhibitor, on iliac artery stenosis after balloon injury in rabbits
被引：1
|作者：
Fang, Xiao Yi
[2
]
Lin, Ni Yang
[2
]
Li, Yu Guang
[1
]
机构：
[1] Shantou Univ, Affiliated Hosp 1, Dept Cardiol, Coll Med, Shantou 515041, Peoples R China
[2] Shantou Univ, Affiliated Hosp 1, Dept Pediat, Coll Med, Shantou 515041, Peoples R China
关键词：
sodium-hydrogen exchanger;
amiloride;
balloon injury;
restenosis;
vascular smooth muscle cell;
cellular proliferation;
percutancous transluminal coronary angioplasty;
VASCULAR SMOOTH-MUSCLE;
GROWTH-FACTOR;
CORONARY-ARTERIES;
PORCINE CORONARY;
GENE-EXPRESSION;
CELL-MIGRATION;
PROLIFERATION;
ANTIPORTER;
HYPERTROPHY;
ACTIVATION;
D O I：
10.1139/O10-003
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
This study was designed to explore the effects of amiloride, a Na+-H+ exchange (NHE) inhibitor, on vessel stenosis by observing the expression of NHE-1 protein in vascular smooth muscle (VSM) after balloon injury and the effects of amiloride on VSM cell proliferation, migration, and excretion of extracellular matrices (ECMs). A total of 32 adult male New Zealand white rabbits were randomly divided into a balloon injury group (BG), an amiloride-treated group (AG). and a sham-operated group (SG) The left iliac artery was injured by Inflating a 2.5 mm x 20 mm Foley catheter in BG and AG rabbits, in SG rabbits, the Foley catheter was inserted but not inflated. Amiloride (5 mg kg(-1).d(-1)) was injected intraperitoneally in AG and the same volume of distilled water was used in BG 3 clays before balloon injury and for 28 days after the injury The left iliac artery was stained by hematoxylin-cosin, a-actin. and Masson's trichrome to observe the vessel cava. neointima. media layer, and ECMs. NHE-1 proteins of the VSM were detected by Western blotting A narrowing of the arterial cava, neointima formation, and thickened VSM layer were observed 28 days after balloon injury in BG and AG However, in AG, the vessel cava was not as narrowed as that of BG and the intimal areas were to a lesser extent than in BG In AG. the a-actin-positive areas and the ECM areas in the neointima were increased compared with SG. but to a lesser extent than in BG The expression of NHE-1 protein in VSM was increased in BG and AG after balloon injury, however, the levels in AG were significantly less than in BG In conclusion, VSM cell proliferation, migration. and excretion of ECMs contributed to vessel stenosis in the BG and AG rabbits The expression of NHE-1 protein in VSM increased after balloon injury Amiloride, an inhibitor of NHE-1, can limit the development of vessel stenosis through inhibition of VSM cell proliferation, migration, and excretion of ECMs
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页码：665 / 670
页数：6
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