Chromatographic purification of serum and colostral immunoglobulins of camel (Camelus dromedarius)

Boric acid and ammonium sulphate precipitated serum globulins were used to obtain IgM from sephadex G-200 column eluted with 0.01 M phosphate buffer saline, pH 7.5. The boric acid precipitation gave better yield. The IgG(2) was isolated from ammonium sulphate precipitated serum globulins from DEAE-cellulose column eluted with 0.01 M phosphate buffer without sodium chloride gradient. The IgG(2) was isolated from ammonium sulphate precipitated colostral globulins by a combination of both sephadex G-200 and DEAE-cellulose chromatography. The traces of IgG(2) from IgG(1) rich eluates were removed by S. aureus protein A absorption to obtain pure IgG(1). S. aureus antigen was also used to identify eluates containing IgG(2).