Full-length cDNA cloning of human neuroglobin by using electronic sequence elongation technique and RACE technique

Human neuroglobin (NGB) is a newly-discovered gene functioned specifically in the oxygen supply and oxygen consumption in the brain. However, the full-length cDNA sequence of NGB was not reported yet. Using the in silicon sequence elongation technique, 5'-RACE and 3'-RACE technique, the full-length cDNA sequence of human neuroglobin was successfully obtained (GenBank accession number AF422797). The cDNA sequence of human NGB is 1 909 bp in size and capable of encoding a protein of 151 amino acids. The 5'-untranslated region is 375 bp, while the 3'-untranslated region is 1078 bp including 27 bp of poly (A) sequence. Results demonstrated that the RACE technique coupled with electronic sequence elongation technique is useful to obtain unknown sequence of 5'-terminal or 3'-terminal part of a new gene.