Application of rank annihilation factor analysis to the determination of the stability constant of the complex XL and rate constants for the reaction of x and XL with the reagent Z using kinetic profiles

A simple procedure for the kinetic spectrophotometric determination of the formation constant of 1:1 (XL) complexes (or adducts) using rank annihilation factor analysis (RAFA) is proposed. The method was based on the effect of the concentration of L (or X) on the reaction of X (or Q with a reagent (Z). The rate constants for the reactions of X (or L) and XL with Z were also determined. In order to perform RAFA, concentration profiles were calculated by optimizing the value of formation constant. The rank of the original data matrix was reduced by one by annihilating the information of X (or L). The best estimation of rate constant of complex XL reduced the rank of the system to zero (noise level). The performance of the method was evaluated using synthetic data as well as experimental data, and good results were obtained. The spectrophotometric data for the study of the effect of beta-cyclodextrin ( beta-CD) on the oxidation reaction of some catechol derivatives with iodate at pH 3.60 was used as an example. The difference in the rate of the oxidation reaction of catechol derivatives with iodate at pH 3.60 in the presence of different concentrations of beta-CD was monitored.