Protein-mesoporous silicon matrix obtained by S-layer technology

Protein layers on porous silicon (PS) substrates were prepared using haloarchaea strain Haloferax sp. as S-layer subunits. The attaching of S-layer at PS samples was performed by immersing the PS samples in S-layer solution in sterile conditions, followed by 24 hours incubation at two temperatures, 4 and 24 degrees C. Spectrophotometric determination of the S-layer attachment on the porous silicon substrate was performed at 280 nm wavelength by measuring the protein concentration in solution before and after the incubation of PS samples. The protein layer morphology on the PS substrate was investigated by electron microscopy. (C) 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim