Encapsulation of Fish Oil Into Low-Cost Alginate Beads and EPA-DHA Release in a Rumino-Intestinal In Vitro Digestion Model

被引:15
|
作者
Olloqui, Enrique J. [1 ]
Castaneda-Ovando, Araceli [1 ]
Contreras-Lopez, Elizabeth [1 ]
Hernandez-Sanchez, David [2 ]
Tapia-Maruri, Daniel [3 ]
Piloni-Martini, Javier [4 ]
Anorve-Morga, Javier [1 ]
机构
[1] Univ Autonoma Estado Hidalgo, Dept Chem, Carr Pachuca Tulancingo Km 4-5, Mineral De La Reforma 42184, Hidalgo, Mexico
[2] Colegio Posgrad, Programa Ganaderia, Campus Motecillo,Carr Mexico Texcoco,Km 36-5, Texcoco 56230, Estado De Mexic, Mexico
[3] Inst Politecn Nacl, Area Microscopy, Ctr Desarrollo Prod Biot, Carretera Yautepec Jojutla,Km 6 Calle CEPROBI 8, Yautepec 62731, Morelos, Mexico
[4] Univ Autonoma Estado Hidalgo, Dept Vet & Zootech, Av Univ Km 1 Ex Hda Aquetzalpa AP 32, Tulancingo 43600, Hidalgo, Mexico
关键词
alginate; beads; DHA; encapsulation; EPA; ruminant; POLYUNSATURATED FATTY-ACIDS; RUMEN BIOHYDROGENATION; LIVER OIL; DIGESTIBILITY; LIPOLYSIS; QUALITY; MILK;
D O I
10.1002/ejlt.201800036
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Fish oil-origin eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are encapsulated with formulated alginate to prevent biohydrogenation in the rumen. Beads are formulated with 0.3%, 1.0%, and 2.0% of alginate. Bead surface poreformation is characterized by environmental scanning electron microscopy (ESEM). Observed pore sizes according to alginate concentration are 17-33 mu m (0.3%), 1018 mu m (1.0%), and 5-10 mu m (2.0%). Release of EPA and DHA is evaluated during ruminal, abomasal, and intestinal simulated digestion. Beads shows high percentage of encapsulation efficiency (91.39% from EPA and 93.51% from DHA). Beads designed (1.0%) allows to obtain EPA release of 13.65% (ruminal digestion), 29.07% (abomasal digestion), and 28.21% (intestinal digestion); while, DHA releases under the same conditions are: 18.55%, 26.78%, and 43.16%, respectively. Beads with 1.0% of alginate had the best release pattern during ruminal simulated digestion allowing an increased intestinal availability of EPA and DHA. Practical Applications: Fish oil encapsulation into alginate beads is a good alternative for ruminant diets supplementation, which will allow to get animal origin food (milk and meat, mainly) enriched with essential fatty acids such as EPA and DHA.
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页数:9
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