Optimization study for sorbitol production by Zymomonas mobilis in sugar cane molasses

被引:48
|
作者
Cazetta, ML
Celligoi, MAPC
Buzato, JB
Scarminio, IS
da Silva, RSR
机构
[1] Londrina Stat Univ, Dept Food Technol, BR-86051990 Londrina, Parana, Brazil
[2] Londrina Stat Univ, Dept Biochem, BR-86051990 Londrina, Parana, Brazil
[3] Univ Estadual Londrina, Dept Chem, BR-86051990 Londrina, Parana, Brazil
关键词
sorbitol; Zymomonas mobilis sugar cane molasses; response-surface methodology;
D O I
10.1016/j.procbio.2004.01.041
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sorbitol production by Zymomonas mobilis in sugar cane molasses was investigated by batch fermentation. The effects of the mean sugar concentration, temperature, agitation, and culture time were studied simultaneously by factorial analysis. Maximum sorbitol production was determined using a second-order central composite design and analyzed by the superficial response method. Response-surface methodology analysis showed that the best conditions for sorbitol production were 300 g/l total reducing sugars (TRS) in the culture medium, 36 h at 35degreesC fermentation time. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:747 / 751
页数:5
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