Conjugative IncX1 Plasmid Harboring Colistin Resistance Gene mcr-5.1 in Escherichia coli Isolated from Chicken Rice Retailed in Singapore

被引：20
|
作者：
Guo, Siyao [1 ,2 ]
Tay, Moon Y. F. [1 ,2 ]
Thu, Aung Kyaw [1 ,2 ,4 ,5 ]
Seow, Kelyn Lee Ghee [1 ,2 ]
Zhong, Yang [1 ,2 ]
Ng, Lee Ching [3 ,4 ]
Schlundt, Joergen [1 ,2 ]
机构：
[1] Nanyang Technol Univ, Food Technol Ctr NAFTEC, Singapore, Singapore
[2] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore
[3] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore
[4] Natl Environm Agcy, Environm Hlth Inst, Singapore, Singapore
[5] Singapore Food Agcy, Natl Ctr Food Sci, Singapore, Singapore
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2019年 / 63卷 / 11期
关键词：
Singapore; antimicrobial resistance; colistin resistance; food safety; mcr gene; plasmids;
D O I：
10.1128/AAC.01043-19
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Colistin is regarded as one of the last-resort antimicrobials for treatment of Gram-negative bacterial infections (1). Several cases of plasmid-borne colistin resistance genes mcr-1, mcr-3-like, and mcr-4.2 in clinical Enterobacteriaceae isolates, including Escherichia coli, have been reported in Singapore (2-4). However, the mcr-5 gene has not been reported in clinical isolates in Singapore. Previously, we reported the antimicrobial resistance (AMR) genotype and phenotype of E. coli SGEHI2010ENV103 isolates from ready-to-eat food in Singapore and documented the first isolate carrying mcr-5.1 in Singapore (5). In this study, we further analyzed this isolate. Here, we report the first complete nucleotide sequence of a transferable plasmid harboring mcr-5.1 in E. coli isolated from ready-to-eat chicken rice in Singapore. (Chicken rice is a common dish in Singapore which is composed of cooked chicken and seasoned rice, served with sauce and cucumber garnishes). © 2019 American Society for Microbiology. All Rights Reserved.
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页数：4
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