Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF

被引：10
|
作者：
Rindi, Laura [1 ]
Ali, Greta [2 ]
Fabiani, Barbara [1 ]
Fontanini, Gabriella [3 ,4 ]
Garzelli, Carlo [1 ]
机构：
[1] Univ Pisa, Dept Translat Res & New Technol Med & Surg, I-56127 Pisa, Italy
[2] Univ Hosp Pisa, Unit Pathol Anat, I-56126 Pisa, Italy
[3] Univ Pisa, Dept Surg Med & Mol Pathol, I-56126 Pisa, Italy
[4] Univ Pisa, Crit Area, I-56126 Pisa, Italy
来源：
TUBERCULOSIS | 2017年 / 106卷
关键词：
REAL-TIME PCR; XPERT MTB/RIF; EXTRAPULMONARY TUBERCULOSIS; PULMONARY TUBERCULOSIS; DIAGNOSTIC-ACCURACY; ASSAY; COMPLEX; METAANALYSIS; RESISTANCE; BONE;
D O I：
10.1016/j.tube.2017.06.005
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：53 / 55
页数：3
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