Spontaneous gallbladder perforation is a very rare condition in infants, Most gallbladder perforations occur in adults, frequently due to trauma. Ultrasound, peritoneal lavage, and retrograde cholangiography may give indirect evidence of the possibility of this condition. There is no definitive diagnostic modality that can definitely determine the diagnosis in such cases. A case of gallbladder perforation with localized biliary ascites detected on radionuclide hepatobiliary imaging is presented.